Coding

Part:BBa_K1199042:Design

Designed by: Tian Wang   Group: iGEM13_UESTC_Life   (2013-09-15)

LinB 1,3,4,6-tetrachloro-1,4-cyclohexadiene->2,5-dichloro-2,5-cyclohexadiene-1,4-diol


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 157
    Illegal NgoMIV site found at 648
    Illegal NgoMIV site found at 682
    Illegal AgeI site found at 82
    Illegal AgeI site found at 748
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

No restriction sites contained


Source

LinB is from Pseudomonas paucimobilis UT26

References

1.Nagata Y, Natsui S, Endo R, Ohtsubo Y, Ichikawa N, Ankai A, Oguchi A, Fukui S, Fujita N, Tsuda M.Genomic organization and genomic structural rearrangements of Sphingobium japonicum UT26, an archetypal γ-hexachlorocyclohexane-degrading bacterium.2011 Dec 10;49(6-7):499-508. doi: 10.1016/j.enzmictec.2011.10.005. Epub 2011 Nov 7.